| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088199 |
| Chromosome: | chromosome 10 |
| Location: | 2198124 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGAATGGCGGCAGTAGCATCCAGGAGT |
| Internal bar code: | TAAAATATGGGTTGGACCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1289 |
| LEAP-Seq percent confirming: | 99.5765 |
| LEAP-Seq n confirming: | 7054 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGTGCTGCAACCATTA |
| Suggested primer 2: | CTACACCTGTGGTGGCTGTG |