Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.088204 |
Chromosome: | chromosome_2 |
Location: | 1082852 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g081000 | EXN4 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGAGTGCCAGTCCTTGTTCAGGTCGGCCT |
Internal bar code: | GGGCACGGTCTTGCAAAGGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 663 |
LEAP-Seq percent confirming: | 98.8264 |
LEAP-Seq n confirming: | 3200 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTACAGGGTTGTAGGGGGT |
Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |