| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088204 |
| Chromosome: | chromosome_2 |
| Location: | 1082858 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g081000 | EXN4 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTGACTCGTGGCGACGCCAACAATCTCG |
| Internal bar code: | TCTAGACAACTAGTGTGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.5171 |
| LEAP-Seq n confirming: | 2473 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACAGGGTTGTAGGGGGT |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |