| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088204 |
| Chromosome: | chromosome_13 |
| Location: | 4165425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g592050 | DAL1 | Allantoinase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTGAGGTAGTGTGCGCACGTCTCTACAGT |
| Internal bar code: | ACTAGTTGCAGGCGGCTTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 98.4117 |
| LEAP-Seq n confirming: | 9728 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGCTCGCCATCACAAGT |
| Suggested primer 2: | CTGTCCTGTTCTCCTCAGCC |