| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088225 |
| Chromosome: | chromosome 1 |
| Location: | 4057126 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026500 | ASP3 | (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGCCGGACTTGGGGCGGGCGTGATTT |
| Internal bar code: | GGTCCGCGGACGCTGGGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 98.7355 |
| LEAP-Seq n confirming: | 1874 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTCGACAACGCCTAC |
| Suggested primer 2: | CAGGTGTTTGTATTGGTGCG |