| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088225 |
| Chromosome: | chromosome 8 |
| Location: | 4450278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382250 | (1 of 4) PTHR11584//PTHR11584:SF377 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATTGCGACAGTGGGATGCCTTCAGGA |
| Internal bar code: | CATTGGCCGGCCAGGCGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 97.2477 |
| LEAP-Seq n confirming: | 1272 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCACTCTTACTTCCGGC |
| Suggested primer 2: | TGGCCTCATCACTCTCACAG |