| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088248 |
| Chromosome: | chromosome 17 |
| Location: | 3506439 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTTGAGTTCCACGAGTCCACACAATG |
| Internal bar code: | CCGCTGCTAAGGATCCACGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.3108 |
| LEAP-Seq n confirming: | 3026 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAAGGCATTTTAAGCGA |
| Suggested primer 2: | TTGTGTGCTTTGGATTCGAC |