| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088252 |
| Chromosome: | chromosome 13 |
| Location: | 3246801 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585850 | b-1%252C4-N-acetylglucosaminyl transferase; (1 of 1) 2.4.1.141 - N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTGCATCATGATGCAGTACAGCCCTC |
| Internal bar code: | TGAATTGTTGAGTCCGATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.5463 |
| LEAP-Seq n confirming: | 2194 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGAGACACACATCCACA |
| Suggested primer 2: | AGGTCAGGACGCAGTTGTCT |