| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088272 |
| Chromosome: | chromosome 2 |
| Location: | 2393463 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091200 | (1 of 1) 1.13.11.5 - Homogentisate 1,2-dioxygenase / Homogentisicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCTGCACGGGACACCCGGGCTATTTGC |
| Internal bar code: | ACACAAGCTGCAAGGTGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 97.9681 |
| LEAP-Seq n confirming: | 3375 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCATGTTGGAGTTCTGCG |
| Suggested primer 2: | TACTCCTCACAGGGGGTGTC |