| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088279 |
| Chromosome: | chromosome 17 |
| Location: | 538425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699550 | (1 of 37) IPR008979 - Galactose-binding domain-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGAGTGAGGCTGGCGCGCGGCTTGAAA |
| Internal bar code: | TCGAACTTTAAAAACAAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1170 |
| LEAP-Seq percent confirming: | 99.2922 |
| LEAP-Seq n confirming: | 2525 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCATACACCGAGAAGGT |
| Suggested primer 2: | TGAGCCACGTCACAACTAGC |