| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088284 |
| Chromosome: | chromosome_9 |
| Location: | 2144438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g393450 | FAP233 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTATGGGCAGGTGGCAAACTCAGAAGTGA |
| Internal bar code: | GTCCGCAGTGGCCACCCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 99.6381 |
| LEAP-Seq n confirming: | 826 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGTCGTGTTGCCCAAAT |
| Suggested primer 2: | TGACGTAGTCGGCGTAGTTG |