| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088300 |
| Chromosome: | chromosome 6 |
| Location: | 2136657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265652 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCTGCTGTTGCTGCCGCTGGTGCCCT |
| Internal bar code: | GAATCACTGGACGGCCGAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.8739 |
| LEAP-Seq n confirming: | 4752 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACATCAGGTCCAAGGTC |
| Suggested primer 2: | AAACAAGCACCCTGAACCAC |