| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088319 |
| Chromosome: | chromosome_1 |
| Location: | 2905619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g017650 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCTCGGAGAGCAAATGCTGGGGCTGGAGC |
| Internal bar code: | CGCTCACTTGTGGCGGGACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.184 |
| LEAP-Seq n confirming: | 2431 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACAGGCCATGCTCTTC |
| Suggested primer 2: | TTCGTGGCTGAGCTTGTATG |