Insertion junction: LMJ.RY0402.088342_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.088342
Chromosome:chromosome 2
Location:7115558
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g146851 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGAGGTTTACTGCTCGAGTACAGTCGT
Internal bar code:GCGTAACGTTGTCCTGGTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:655
LEAP-Seq percent confirming:96.5019
LEAP-Seq n confirming:1269
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTGGCTTTTGGACCACT
Suggested primer 2:CATTTCAGCGACAGGACTCA