Insertion junction: LMJ.RY0402.088357_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.088357
Chromosome:chromosome 6
Location:2676998
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270200 PLC1,PLC Phospholipase C; (1 of 1) 3.1.4.11 - Phosphoinositide phospholipase C / Triphosphoinositide phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACAGCTTCCTTGCCTTTGCCCAGGTGG
Internal bar code:TTCGATCCAAAGTGGTCGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:658
LEAP-Seq percent confirming:99.2918
LEAP-Seq n confirming:701
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACGCCAACAAATGAATG
Suggested primer 2:CGCTGGAGAAGAGTACTGGG