| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088372 |
| Chromosome: | chromosome 13 |
| Location: | 1344237 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571450 | (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTATGACGCAGCGGGATAGCGATGAGA |
| Internal bar code: | TGGAGGCCTTGGAGGTATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.1389 |
| LEAP-Seq n confirming: | 5066 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCAAGACATTGGACGA |
| Suggested primer 2: | CAGATGGTGGTGTGAACCTG |