| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088375 |
| Chromosome: | chromosome 12 |
| Location: | 3913103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515950 | (1 of 35) IPR001763 - Rhodanese-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGATGGGCGCTGGAGTCGCGGCCATTGG |
| Internal bar code: | CCCCTTCTGATTGCGTGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 98.9669 |
| LEAP-Seq n confirming: | 10633 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGTCCTGAGTGGTGGT |
| Suggested primer 2: | AGCCGCAAATGGAATACAAC |