| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088393 |
| Chromosome: | chromosome 4 |
| Location: | 1422638 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214250 | AGO2 | Argonaute-like protein; (1 of 3) PTHR22891:SF0 - PROTEIN ARGONAUTE-2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAAGTAGCGGCCATGTCGGGCCGAGG |
| Internal bar code: | ATGTGATCGTACAACCAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 98.2093 |
| LEAP-Seq n confirming: | 5978 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTCACTGCCATGAGTA |
| Suggested primer 2: | ATGATTACGCATGGATGCAA |