| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088394 |
| Chromosome: | chromosome 6 |
| Location: | 4052986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278210 | STA5,PGM,GPM1,PGM1 | Phosphoglucomutase 1; (1 of 1) PTHR22573//PTHR22573:SF2 - PHOSPHOHEXOMUTASE FAMILY MEMBER // PHOSPHOGLUCOMUTASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTTCGGCGCCTCACATTGATGGTGCGC |
| Internal bar code: | GGCCCTTGTGGGCGCTACCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.4424 |
| LEAP-Seq n confirming: | 3032 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTAAGGTGAAATGCGTC |
| Suggested primer 2: | CAAATGGATGCTGTGGTGAC |