Insertion junction: LMJ.RY0402.088396_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.088396
Chromosome:chromosome 6
Location:6087145
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g289800 CAM12 Calmodulin-like protein; (1 of 1) 2.3.1.23//2.3.1.51//2.3.1.67 - 1-acylglycerophosphocholine O-acyltransferase / Lysolecithin acyltransferase // 1-acylglycerol-3-phosphate O-acyltransferase // 1-alkylglycerophosphocholine O-acetyltransferase / Platelet-activating factor-synthesizing enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTTGCCTCCGCCGCTGTGTGCCCTGTG
Internal bar code:GGGAGTGATCAATTAGATCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:95.353
LEAP-Seq n confirming:2688
LEAP-Seq n nonconfirming:131
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCTCTAAGCCTAATGCC
Suggested primer 2:TGCTGGCAGAGATACAGGTG