| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088400 |
| Chromosome: | chromosome 13 |
| Location: | 4952378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606300 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACCATGCAAGCAGGAGTTGACACTGA |
| Internal bar code: | TTCCGATGTAGGAAATGTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.3072 |
| LEAP-Seq n confirming: | 860 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCTCCCACTTTGAAACA |
| Suggested primer 2: | GATCGTGTTCATAATGGGGG |