| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088404 |
| Chromosome: | chromosome_8 |
| Location: | 1127359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362650 | IFT140 | Intraflagellar transport protein 140 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGATACAACGGGGGCCTGCGCAGCAGCGT |
| Internal bar code: | GTAAGAATGCACACTTGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.447 |
| LEAP-Seq n confirming: | 11688 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGTCCAAGGTTATTGGC |
| Suggested primer 2: | ACTTGGAGCTGCTGTGTGTG |