| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088419 |
| Chromosome: | chromosome_8 |
| Location: | 1732665 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g366101 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAATCCACACCAATACACACCCACCCACCA |
| Internal bar code: | GCACGCGCAAAAAATGCGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTTCCAACAGAAGTAGC |
| Suggested primer 2: | GTTCTTGCTTGTGTGCGTGT |