Insertion junction: LMJ.RY0402.088419_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.088419
Chromosome:chromosome 8
Location:1732671
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g366101 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAAGACGTGCGCATGTGCGCGCGCTAA
Internal bar code:TTCTTTACAATTTCATTTATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:901
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCTTCCAACAGAAGTAGC
Suggested primer 2:GTTCTTGCTTGTGTGCGTGT