Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.088430 |
Chromosome: | chromosome 1 |
Location: | 1859421 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g010150 | (1 of 3) IPR006073//IPR027417 - GTP binding domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAAATGAAGCGGCGGTGCCGCGGGAA |
Internal bar code: | GGGAAAGGACGCGCAAATATTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 224 |
LEAP-Seq percent confirming: | 98.7937 |
LEAP-Seq n confirming: | 819 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTCAATCGCCTGCTTACA |
Suggested primer 2: | TGGAAACGCAAACACACATT |