| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088474 |
| Chromosome: | chromosome 11 |
| Location: | 3563694 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481700 | SPL17 | Pre-mRNA splicing factor; (1 of 1) PTHR13161:SF15 - SPLICING FACTOR, SUPPRESSOR OF WHITE-APRICOT HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGGAGTGAACGGGTGCACCCTTGGGT |
| Internal bar code: | GGCGGCAGATCTGTCGACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 97.2949 |
| LEAP-Seq n confirming: | 4316 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACGGTGGAAACAAAAAG |
| Suggested primer 2: | CTTCGGGCTAGGACTCACTG |