| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088484 |
| Chromosome: | chromosome 3 |
| Location: | 5076463 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGGGTAGCCCTGCCACACTAGTGCCCC |
| Internal bar code: | AGCAAATCATGTAAATAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1322 |
| LEAP-Seq percent confirming: | 99.5516 |
| LEAP-Seq n confirming: | 6217 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCCCGACTTGAACAGC |
| Suggested primer 2: | TTAGTGCTGTCAGTGACCCG |