| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088485 |
| Chromosome: | chromosome 12 |
| Location: | 249272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGTAGTCTCAGGCGGCAAGCGTCTAC |
| Internal bar code: | CTTTGTGCGAATTCAATACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.1279 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTAGAGGGCGCTAACGTG |
| Suggested primer 2: | CAGGCCTTCTCTGACGGTAG |