Insertion junction: LMJ.RY0402.088488_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.088488
Chromosome:chromosome 7
Location:4109470
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCAGCCCGATGGCCCCACGGCACGGCGG
Internal bar code:GAAGCGGAACATTGACCCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:707
LEAP-Seq percent confirming:98.3823
LEAP-Seq n confirming:1642
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCTGTCCGTGTTTC
Suggested primer 2:AAACTGAATCGAACCCAACG