| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088495 |
| Chromosome: | chromosome 1 |
| Location: | 2011344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010832 | (1 of 1) PTHR12411:SF389 - CATHEPSIN L1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACAGCTACTGCTACTGCCGAGCAGCAG |
| Internal bar code: | GGGTGTGTCGCGAGCGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 80.3927 |
| LEAP-Seq n confirming: | 5527 |
| LEAP-Seq n nonconfirming: | 1348 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGAGTGGAATGTCAGGG |
| Suggested primer 2: | TTTTCCATCATCAGGGCTTC |