Insertion junction: LMJ.RY0402.088497_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.088497
Chromosome:chromosome_9
Location:5020454
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g398104 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGCTCCTCCGATAGTTTCGACAGCGTAAA
Internal bar code:CTGGGCGGCGCCGCTCGTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:98.978
LEAP-Seq n confirming:2518
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCCGAGGTAAGGGTATG
Suggested primer 2:CAGTCTGAGTCTAGCGCACG