| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088527 |
| Chromosome: | chromosome 7 |
| Location: | 4967450 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346698 | PHC75 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTACGTGGATGAGCGAGAGTGTGTGA |
| Internal bar code: | TCGCCTAAACCTACCTCGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7734 |
| LEAP-Seq n confirming: | 1761 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCTCCTCTCGTTCCTT |
| Suggested primer 2: | GTATCCGTTTGCTCGGATTG |