| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088541 |
| Chromosome: | chromosome 2 |
| Location: | 5722908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110150 | (1 of 1) PF00651//PF03110//PF07707 - BTB/POZ domain (BTB) // SBP domain (SBP) // BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGGCGGCAGCTTTGCCATGAATGCCTC |
| Internal bar code: | GCACGAGTTCATAGACGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 99.6288 |
| LEAP-Seq n confirming: | 9395 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAACCAAGTCTTGCCAC |
| Suggested primer 2: | ATGCATTCATTCAGCAGCAG |