Insertion junction: LMJ.RY0402.088572_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.088572
Chromosome:chromosome 1
Location:1560495
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g008100 (1 of 34) IPR000571 - Zinc finger, CCCH-type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTATCACCTGATGCTTGCGTCCGTCA
Internal bar code:GGATACGATAAACTAAATACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:645
LEAP-Seq percent confirming:98.2379
LEAP-Seq n confirming:446
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCCTCAAGACTAAGCG
Suggested primer 2:TCACCTTGAAGCTGTGCATC