Insertion junction: LMJ.RY0402.088594_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.088594
Chromosome:chromosome 6
Location:4103686
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278220 ELG38 Exostosin-like glycosyltransferase 38; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCTTAAGTAACCATGGGTGTAAATCCC
Internal bar code:GTAACGAGGTATTCAGGGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:625
LEAP-Seq percent confirming:99.7131
LEAP-Seq n confirming:3823
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACTTGGACGACACCCAGTC
Suggested primer 2:TGTTCACCATTCCGATGAGA