| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088629 |
| Chromosome: | chromosome_17 |
| Location: | 511913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g699500 | TTL8,TTLL9,FAP267 | Tubulin tyrosine ligase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCTTGAGGTTGGCGTCAATCATGACGTC |
| Internal bar code: | GACCCCGACCGCATTTCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 96.5157 |
| LEAP-Seq n confirming: | 831 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGGTTGGAGAGGTGTT |
| Suggested primer 2: | ATATGCTCTTGTTGGGGTGC |