Insertion junction: LMJ.RY0402.088629_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.088629
Chromosome:chromosome 17
Location:511913
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g699500 TTLL9,Tpg1,TTL8,FAP267 (1 of 1) K16603 - tubulin polyglutamylase TTLL9 [EC:6.-.-.-] (TTLL9); Tubulin-Tyrosine Ligase 9 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTTGAGGTTGGCGTCAATCATGACGTC
Internal bar code:GACCCCGACCGCATTTCGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:481
LEAP-Seq percent confirming:96.5157
LEAP-Seq n confirming:831
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGGTTGGAGAGGTGTT
Suggested primer 2:ATATGCTCTTGTTGGGGTGC