| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088653 |
| Chromosome: | chromosome_17 |
| Location: | 1148704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g704300 | FAP47 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGGAGCCTGGGGGCGAGGGGCAGGACGT |
| Internal bar code: | GCTGTTCTATTAGGCCCCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 97.4576 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTAGCTGATGACGTAG |
| Suggested primer 2: | GTATGCGGGAAGTTGACGTT |