Insertion junction: LMJ.RY0402.088671_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.088671
Chromosome:chromosome 7
Location:6096914
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g355466 AGE2 DNA repair glycosylase; (1 of 1) PF15628 - RRM in Demeter (RRM_DME) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTGCCTGCTGGAGTGCCTCCGACCCG
Internal bar code:GTGATGTCATGTTGCTGCGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:255
LEAP-Seq percent confirming:99.1051
LEAP-Seq n confirming:1329
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGGCACGATCAATCAAG
Suggested primer 2:GCTTCAACGACTTCCTGACC