| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088688 |
| Chromosome: | chromosome 3 |
| Location: | 1129811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149100 | CIS2 | Citrate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATTGAGCAGAGTAGGAACAGGCAAACA |
| Internal bar code: | ATTGAGGGGTCAGGGCAGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.2436 |
| LEAP-Seq n confirming: | 3149 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGCAATGCGATGTTAT |
| Suggested primer 2: | CTGGAAGACCCAAACATCGT |