| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088713 |
| Chromosome: | chromosome_9 |
| Location: | 3232659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g387689 | FAP76 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TATGCCGCAAAGGTGCGAGCGGCGTGCGCG |
| Internal bar code: | GGCGTGTGGAATACGTCCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 98.32 |
| LEAP-Seq n confirming: | 1229 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGCGGTGCGATTACACA |
| Suggested primer 2: | GGAGGACATGAAGGAGGTGA |