| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088714 |
| Chromosome: | chromosome 16 |
| Location: | 6881943 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676421 | TOC1,PRR1 | Pseudo Response Regulator 1; (1 of 1) PTHR26402//PTHR26402:SF465//PTHR31319 - RESPONSE REGULATOR OF TWO-COMPONENT SYSTEM // TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR1-RELATED // FAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGCCGACAGGCTCGGTGCGCCAGTGG |
| Internal bar code: | GACCGCTTGGTGCACGCGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.7941 |
| LEAP-Seq n confirming: | 1454 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTATCCAAATGCATCGC |
| Suggested primer 2: | AGTGTCAACCCGTCTCATCC |