| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088719 |
| Chromosome: | chromosome 14 |
| Location: | 2939837 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627650 | FAP392 | Flagellar Associated Protein 392 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCGTCGTCTGGGCGGGCTTGCGTTTC |
| Internal bar code: | CGGGACCAGGAGGTGGCGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 52.7727 |
| LEAP-Seq n confirming: | 866 |
| LEAP-Seq n nonconfirming: | 775 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCTGTGGACTGTTGAT |
| Suggested primer 2: | CTGTGGTGTGGAAAGGTGTG |