| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088745 |
| Chromosome: | chromosome 10 |
| Location: | 4486271 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451752 | PHC25 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACTCACCGCCGTTGCCCAGGGCCCAT |
| Internal bar code: | CAGTAGAGGTTCCCTTTTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.1677 |
| LEAP-Seq n confirming: | 834 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGGGCAGTACTTGGTG |
| Suggested primer 2: | GGGCTGAGATGTAGGCTGAG |