| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088758 |
| Chromosome: | chromosome 12 |
| Location: | 7940588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552400 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTCTGTCAGCGGCGAGCTGCTACCGG |
| Internal bar code: | CGTGGAGCTCATCATGCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1213 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTATCCTGTCCCCGTAA |
| Suggested primer 2: | AGAAATGCTTGGGGTGTTTG |