| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088766 |
| Chromosome: | chromosome_12 |
| Location: | 8873086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g544700 | PUF3 | Puf protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTTCGTCTAGGGCGTGACCAGTCGACCTG |
| Internal bar code: | CATAGTTAGCAGTTCGTTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.3056 |
| LEAP-Seq n confirming: | 572 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAACGCGGGACTTACCAA |
| Suggested primer 2: | AGCTGGCCCCAGGTACTTAT |