Insertion junction: LMJ.RY0402.088773_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.088773
Chromosome:chromosome 9
Location:4834146
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g397512 HSPBP1 hsp70-binding protein 1; (1 of 1) K09562 - hsp70-interacting protein (HSPBP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAGAAGGACCCAGAGGAGCTCAGGAGCA
Internal bar code:GATAATTACCGCGTTGTCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:98.8938
LEAP-Seq n confirming:894
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGGAAACGATGAAGTGG
Suggested primer 2:GACGATAAGAGTCAACCCGC