| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088774 |
| Chromosome: | chromosome 1 |
| Location: | 7850796 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g058886 | (1 of 1) K06944 - uncharacterized protein (K06944) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCCGGGCGCATGCGACACCCCCACAC |
| Internal bar code: | TGAGGTATAATGTGACCCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 86.7257 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGGCCACACTCTCCTC |
| Suggested primer 2: | GGGCGTGAGTGTATGTGTGA |