| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088779 |
| Chromosome: | chromosome 7 |
| Location: | 4873587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346100 | (1 of 1) 3.1.21.7 - Deoxyribonuclease V / Escherichia coli endodeoxyribonuclease V | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCCATGCGCTCTTTTGCGCCCCCGCCT |
| Internal bar code: | GTGTCGGATTGCCGGTGGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 96.2815 |
| LEAP-Seq n confirming: | 7250 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCGTGCGACAGGATTAC |
| Suggested primer 2: | CCCAAACTAAATCGAGCCAA |