| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088794 |
| Chromosome: | chromosome 12 |
| Location: | 5104220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526850 | HEL56 | (1 of 1) K14810 - ATP-dependent RNA helicase DDX56/DBP9 [EC:3.6.4.13] (DDX56, DBP9); DEAD box ATP-dependent RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACGCATGGGCACACAGATGATTCTCC |
| Internal bar code: | GGTGTCTCCCCTCAGGCCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 20 |
| LEAP-Seq percent confirming: | 5.04108 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 2543 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTTGCACGGAGAGCACTT |
| Suggested primer 2: | TCAGCCTCCTCTTCGACATT |