| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088800 |
| Chromosome: | chromosome_10 |
| Location: | 4559636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452400 | CSF1 | Predicted protein of CSF family | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAATCACGCGACCTAGAGTCTTGCCGTAA |
| Internal bar code: | CGCATGGCCTTTTTCCAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 99.581 |
| LEAP-Seq n confirming: | 8080 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGGTTACGACAACACG |
| Suggested primer 2: | CTCAGACCTCAGCCCTTCAC |